Grain Transfers (G2G) & powdered cake... Return To Archives | Search

Please Visit Our New Forums at Mycotopia
Please visit our Sponsors

Mycotopia Web Archive Archive Grain Transfers (G2G) & powdered cake spawn. Previous Next

ClosedClosed: New threads not accepted on this page
Topic Author Last Poster Posts Pages Last Post
Cloning using Dry Tissue - 1
G2GSweetnessSweetness25 1 04-03-04  07:48 pm
rodger's g2g Suckerfree12 2 05-12-04  08:31 pm
using powdered pf [brf] cakes for g2gAdmin (Admin)Admin 135 8 12-19-04  01:54 am
Question about g2g tek?need 4 weedSea of Weed6 1 06-02-03  12:11 am
G2g transfersJack Handyrodger rabbit2 1 06-04-03  11:32 pm
Open air grain to grain transfer idea.. Hippie3faht3 1 08-06-03  02:14 am
G2g problemSpedirikoHippie39 1 08-13-03  03:10 pm
G2gjimmy jublanskiloosid10 1 10-14-03  05:45 am
Metal to metal lid filter Hippie3Joe Millionaire4 1 11-21-03  04:48 pm
Superspawning StrawHippie3Jesse James2 1 12-06-03  05:31 pm
Where'd the myc go?Quarkfaht7 1 02-09-04  01:56 am
G2G pic's - if you never saw anyMycota Hippie3 16 1 01-20-05  02:27 pm

Top of pageBottom of pageLink to this message

Hippie3 (Admin)
Posted on Monday, September 01, 2003 - 03:45 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

Grain to Grain Transfers (G2G)
by
SixTango
Mycota


Quote:

Once you have learned to prepare sterilized spawn pint or quart jars of bird seed, rye or grains & fully colonize them with mycelium. You can easily propagate a single colonized quart jar of that into about 20 more via G2G transfers. Jars propagated via G2G transfers generally colonize under optimal conditions 100% in 10 to 14 days . Given that, spores do not have to germinate, as what is transferred to freshly prepared jars is active mycelium on fully colonized seed or grains.

The method is to prepare fresh jars, just as you would to inoculate via a spore syringe (soak seed, rinse, drain, load, apply filter disk & PC). Excepting, rather than inoculate the fresh jars with a syringe. You transfer grain from a colonized jar to fresh uncolonized jars. The procedure is simple & only requires common sense, minimal preparation, a long stout clean stainless steel spoon & the cleanest personal hygiene and the smallest uncarpeted working place you can muster.

Prepare the smallest cleanest uncarpeted room you have (generally, a bathroom). In the following manner. Clean the room as best you can, getting rid of any dirt, dust, mold or mildew. Remove any cloth hanging anywhere. Spray Lysol on everything, everywhere & wipe it down. If you have any hepa type air filter unit? Place it in the room & run it for at least 1 hour. Running a hepa is preferable, but, if you don't have one. You can usually manage without it

Wipe your fully colonized jar of grain & your fresh jars down, with a clean Lysol sprayed rag. Place those in the room, on the counter top, or whatever flat working surface you intend to use. Wear freshly laundered clean cloths. If you have a face mask (preferable) wear it. If you have a shower cap to cover your hair (preferable), wear it. Enter the room, spray Lysol around (again) run the hepa for a few minutes (if you have one). Then, turn it off. Spray your hands & arms with Lysol & wipe dry.

Unscrew the lid off the colonized jar. Leave the internal filter disk or filter material in place covering the content. Unscrew the lid on a fresh jar, leaving the internal filter material in place. Remove the filter material from the colonized jar & dig up about ¼ of content, as it will be colonized into a solid mass. Spoon out 2 table spoons full & transfer them to the uncolonized jar, by lifting it's filter up & spooning them in. Replace the filter material on the fresh jar IMMEDIATELY after spooning in the colonized material.

Repeat this same process as many times as you have fresh jars to transfer to. Once done. Screw the fresh jars lids on tight. Cover the outside of the lids of the fresh jars with a double layer of alcohol swabbed coffee filters & rubber band them down. Shake each fresh jar to spread the colonized material throughout it. Place your fresh jars in a dry, dark, warm place (preferably between 78 & 84 F), and allow them to colonize in peace & quiet. G2G transfer & shaking jars batters the mycelium. It takes it a day or 3 recuperate from that shock. There is no need to shake G2G jars more than once. As, doing so will only slow colonization, rather than speed it up.
.............................................
.............................................


Top of pageBottom of pageLink to this message

Hippie3 (Admin)
Posted on Monday, September 01, 2003 - 03:48 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)


Quote:

By Nozza (Nozz) on Thursday, November 08, 2001 - 04:20 pm:


If you're innoc'ing with live mycellia then you might want to consider using peroxide for the transfer. You probably know about this already, but if you don't...you just inject about 5-10ml of 3% peroxide into the jar though the lid and shake well, leave to soak in for 1/2 hour and then do your transfer. It works well and provides some extra oxygen as it breaks down...


Top of pageBottom of pageLink to this message

Hippie3 (Admin)
Posted on Monday, September 01, 2003 - 04:08 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

1
2
3
4
5
6
Top of pageBottom of pageLink to this message

Hippie3 (Admin)
Posted on Monday, September 01, 2003 - 04:15 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)


Quote:

When cool, the quart is injected with 12 ccs peroxide, shaken [and then 1/2 hour later opened and] inoculated in front of a flow hood [with some colonized grain or] a piece of peroxidated agar. Colonization is complete in a week with temps around 80 deg F. A huge filter patch bag is prepared with birdseed as above. Peroxide amount is proportional to that listed above. In the flow hood, the bag is inoculated with the colonized quart jar and sealed with an impulse sealer. The bag is colonized in 11 days at room temps.



- mycofile, slightly edited.
Top of pageBottom of pageLink to this message

Hippie3 (Admin)
Posted on Monday, September 01, 2003 - 04:17 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

archive material
Top of pageBottom of pageLink to this message

drugiedugie (Dugzup)
Posted on Monday, September 01, 2003 - 04:23 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

so its saying to shoot your colonized jar with peroxide 1/2 hr prior to doing your transfer to give it more oxygen and does what else?

what are those lids ?
Top of pageBottom of pageLink to this message

Hippie3 (Admin)
Posted on Monday, September 01, 2003 - 04:29 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

the peroxide will kill any contams that might sneak in, making the success of your g2g transfer much more likely.
but it's not your colonized jar you shoot,
it's the fresh uncolonized jars you are preping for the transfer, after they've cooled off.
cool, inject peroxide, shake, sit 30 minutes then open it and spoon in the colonized grain and re-seal.
the lids are plastic, rigged with 6t's pet permanent filter, but polyfil, tyvek and tape will also suffice.
Top of pageBottom of pageLink to this message

Hippie3 (Admin)
Posted on Tuesday, September 02, 2003 - 12:38 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

btw, running one of those ionizers in the work area beforehand is a good idea.
make sure there are no breezes in the work area.
move slowly but efficiently, to minimize disturbing the air.
Top of pageBottom of pageLink to this message

Admin (Admin)
Board Administrator
Username: Admin

Post Number: 8357
Registered: 02-2001
Posted on Tuesday, December 02, 2003 - 07:33 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

by Quincunx

quote:

with g2g transfer fast strains finish it even well under 10 days.

These are my humble Cambodians on day 7 after g2g transfer at 28C (82.4F). They were directly cased app. 30 hrs later...

ratio: 1:75

Rye grain jars (750 mL):



rye

quote:

Pop corn jars (750 mL):



pcorn
Top of pageBottom of pageLink to this message

Admin (Admin)
Board Administrator
Username: Admin

Post Number: 8867
Registered: 02-2001
Posted on Monday, December 08, 2003 - 01:33 pm:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

What is G2G and how is it done?

-------------------------------------------------- ------------------------------

G2G means Grain to Grain transfers.
Once you have learned to prepare sterilized spawn pint or quart jars of bird seed, rye or grains & fully colonize them with mycelium. You can easily propagate a single colonized quart jar of that into about 20 more via G2G transfers.
Spawn rates ranging from 1:10 to 1:40 are being used, with 1:40 being "on the verge of being unsuccessful" [Stamets, GGMM 2000].
Jars propagated via G2G transfers generally colonize under optimal conditions 100% in 10 to 14 days . Given that, spores do not have to germinate, as what is transferred to freshly prepared jars is active mycelium on fully colonized seed or grains.

The method is to prepare fresh jars, just as you would to inoculate via a spore syringe (soak seed, rinse, drain, load, apply filter disk & PC). Excepting, rather than inoculate the fresh jars with a syringe. You transfer grain from a colonized jar to fresh uncolonized jars. The procedure is simple & only requires common sense, minimal preparation, a long stout clean stainless steel spoon & the cleanest personal hygiene and the smallest uncarpeted working place you can muster.

Prepare the smallest cleanest uncarpeted room you have (generally, a bathroom). In the following manner. Clean the room as best you can, getting rid of any dirt, dust, mold or mildew. Remove any cloth hanging anywhere. Spray Lysol on everything, everywhere & wipe it down. If you have any HEPA type air filter unit? Place it in the room & run it for at least 1 hour. Running a HEPA is preferable, but, if you don't have one. You can usually manage without it

Wipe your fully colonized jar of grain & your fresh jars down, with a clean Lysol sprayed rag. Place those in the room, on the counter top, or whatever flat working surface you intend to use. Wear freshly laundered clean cloths. If you have a face mask (preferable) wear it. If you have a shower cap to cover your hair (preferable), wear it. Enter the room, spray Lysol around (again) run the HEPA for a few minutes (if you have one). Then, turn it off. Spray your hands & arms with Lysol & wipe dry.

Unscrew the lid off the colonized jar. Leave the internal filter disk or filter material in place covering the content. Unscrew the lid on a fresh jar, leaving the internal filter material in place. Remove the filter material from the colonized jar & dig up about of content, as it will be colonized into a solid mass. Spoon out 2 table spoons full & transfer them to the uncolonized jar, by lifting it's filter up & spooning them in. Replace the filter material on the fresh jar IMMEDIATELY after spooning in the colonized material.

Repeat this same process as many times as you have fresh jars to transfer to. Once done. Screw the fresh jars lids on tight. Cover the outside of the lids of the fresh jars with a double layer of alcohol swabbed coffee filters & rubber band them down. Shake each fresh jar to spread the colonized material throughout it. Place your fresh jars in a dry, dark, warm place (preferably between 78 & 84 F), and allow them to colonize in peace & quiet. G2G transfer & shaking jars batters the mycelium. It takes it a day or 3 recuperate from that shock. There is no need to shake G2G jars more than once. As, doing so will only slow colonization, rather than speed it up.
Top of pageBottom of pageLink to this message

Admin (Admin)
Board Administrator
Username: Admin

Post Number: 29592
Registered: 02-2001
Posted on Saturday, December 04, 2004 - 12:24 am:Edit Post Quote Text Delete Post Print Post Move Post (Moderator/Admin Only)

btw
one can substitute crumbled pf cake pieces for the grain-to-grain,
making it in effect cake-to-grain
the mycellia will still grow off the crumbled/powdered cake into the grains
quickly.

Namaste


Add Your Message Here
Post:
Bold text Italics Underline Create a hyperlink Insert a clipart image

Username: Posting Information:
This is a private posting area. Only registered users and moderators may post messages here.
Password:
Options: Enable HTML code in message
Automatically activate URLs in message
Action: